13.  Discuss the structure and function of Transfer RNA during the translational process in detail.

answer :

introduction :

tRNA or transfer RNA is a type of RNA, which helps in the synthesis of protein from mRNA. tRNA functions as an adapter molecule during the translation process. It was earlier known as soluble RNA or sRNA. As an adapter, it links the amino acids to nucleic acids. It carries the amino acid to be added in the peptide chain and also deciphers the codon for the same in the mRNA molecule.

Let’s learn about its structure.

tRNA Structure :

The tRNAs are generally 76-90 nucleotides long. Each amino acid has specific tRNAs. Stop codons are not recognised by any tRNAs. The secondary structure of tRNA looks like a clover leaf and tertiary structure is like an inverted ‘L’ shape. The folded structure is formed due to hydrogen bonding between complementary bases.

Also Check: NEET Answer Key

Clover leaf structure of tRNA

The secondary folded structure of tRNA has three hairpin loops, which give it an appearance of three-leafed clover. The main constituents of tRNA are:

Acceptor arm

It is formed by the base pairing of 7-9 nucleotides of 5’ terminal and 3’ terminal. The 5’ terminal has a phosphate group and the 3’ ends with a specific sequence of CCA or CCA tail. The amino acid attaches to the 3’ hydroxyl group of the acceptor arm.

The aminoacylation of tRNA or charging of tRNA is the first step of the translation process. The enzyme aminoacyl tRNA synthetases catalyse the reaction.

DHU Loop

D arm has a stem of 3-4 base pairs and it ends in a loop called D loop as it generally contains dihydrouridine, a modified nucleotide.

Anticodon Loop

It has a 5 base pair long stem. It has an anticodon loop, which contains the complementary codon (3 nucleotides sequence) present on mRNA for the amino acid it carries. These unpaired bases of anticodon loop pair with the mRNA codon. Each codon is identified by a specific tRNA.

TΨC Loop

The T arm consists of a stem of 4-5 bp and a loop containing pseudouridine, modified uridine.

Variable Loop

It is present between the TΨC loop and the anticodon loop. Its size varies from 3-21 bases. It helps in the recognition of the tRNA molecule.

Extended Reading: Difference between mRNA, tRNA and rRNA

tRNA Function

• tRNA plays an important role in protein synthesis.
• It acts as an adapter molecule for linking amino acids to its specific codon present in mRNA.
• Aminoacylation of tRNA is the first step in protein synthesis.
• tRNA is specific to each amino acid and carries them during the translation process in the ribosomal subunits.
• The tRNA transfers the amino acid to the growing polypeptide chain in the ribosomes, which has three binding sites for tRNA, namely A, P and E, which correspond to aminoacyl, peptidyl and exit, respectively.
• This decoding of codons of mRNA by specific tRNAs continues until the entire sequence for a polypeptide chain is translated.

• The prime function of tRNA is to transfer amino acids to form the right sequence of the polypeptides. Hence, it assists protein synthesis.

• It also functions as an adapter molecule to link specific amino acids to the respective codons located in the mRNA molecules.

• Every amino acid defines a particular tRNA. It means that the carriage and transfer of amino acids will not possible without those tRNAs.

• The decoding process of the mRNA continues until the stop codon is reached and not recognized by any of the tRNAs.

conclusion :

This is what you need to know about the structure of this RNA and the tRNA translation process. Understand the complex structure of this transfer RNA molecule and learn how it assists in adding amino acids to the polypeptide chain to build specific proteins. Consider referring to a tRNA diagram to understand the significance of its different sections and base-pair sequences.